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ANDzyme Selection

Our Products: In vitro Selection Kits > ANDzyme Selection
ANDzymesTM  are RNA-cleaving DNAzymes that act upon a proprietary Fluorescent/Quencher substrate to generate a fluorescent signal in the presence of their cognate ligands. In vitro selection of ANDzymeTM is a powerful technique for isolating biosensors with high specificity and sensitivity. The presence of a target ligand will induce a conformational change that will facilitate cleavage of an embedded RNA. Separation of two strands due to this cleavage reaction will cause the ANDzymeTM to fluoresce.  In the ANDzymeTM selection scheme, responsive sequences will yield a cleaved DNA molecule which can be resolved using a denaturing polyacrylamide gel. ANDzymesTM are capable of responding to specific target ligands ranging from metal ions, small molecules, and proteins. 

Features and Benefits:

  • Screen for ANDzymesTM - Fluorescent biosensors targeting metal ions, small molecules and proteins
  • No need for solid-supports for target immobilization or target modification
  • All reagents validated and optimized for in vitro selection
  • Primers designed to maximize enrichment and reduce cross-reactivity
  • Specially designed substrate maximizes fluorescence enhancement while reducing background noise
  • In-depth protocols and guides to avoid common pitfalls and maximize success
  • Directly compatible with Optional Supplementary kits for Next-Generation Sequencing.
    • All ANDzymeTM Starter Kits come with a 10% discount towards NGS primers and sequencing!
NOTE: If you are familiar with the in vitro selection process, our ANDzymeTM Core Library kit will include the necessary components to begin your experiments. If you need any assistance, do not hesitate to contact us. 


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Description
SKU
Price
In vitro Selection Starter Kit for isolating ANDzymes with a 30 nucleotide randomized region
IVSR30K
$495.00
In vitro Selection Starter Kit for isolating ANDzymes with a 40 nucleotide randomized region
IVSR40K
$495.00
In vitro Selection Starter Kit for isolating ANDzymes with a 50 nucleotide randomized region
IVSR50K
$495.00
An initial library with a 30-nucleotide randomized region
IVS30L
$240.00
An initial library with a 40-nucleotide randomized region
IVS40L
$240.00
An initial library with a 50-nucleotide randomized region
IVS50L
$240.00
Additional modified and unmodified primers for amplification of aptamer libraries
IVSPR
$125.00
Fluorescent ladder used in denaturing Polyacrylamide Gel Electrophoresis to monitor library migration
IVSRFL
$120.00
Fluorescent Substrates used by ANDzymes to generate a fluorescent signal in the presence of target molecules
IVSRFQS
$120.00
Standard buffering, salts, and cofactors used for isolating ANDzymes
SB02
$45.00
Individual fluorescent DNA markers used in denaturing Polyacrylamide Gel Electrophoresis
IVSRFM
$40.00