RNA-cleaving DNAzymes that act upon a proprietary Fluorescent/Quencher substrate to generate a fluorescent signal in the presence of their cognate ligands. In vitro selection of ANDzymeTM
is a powerful technique for isolating biosensors with high specificity and sensitivity. The presence of a target ligand will induce a conformational change that will facilitate cleavage of an embedded RNA. Separation of two strands due to this cleavage reaction will cause the ANDzymeTM
to fluoresce. In the ANDzymeTM
selection scheme, responsive sequences will yield a cleaved DNA molecule which can be resolved using a denaturing polyacrylamide gel. ANDzymesTM
are capable of responding to specific target ligands ranging from metal ions, small molecules, and proteins.
Features and Benefits:
- Screen for ANDzymesTM - Fluorescent biosensors targeting metal ions, small molecules and proteins
- No need for solid-supports for target immobilization or target modification
- All reagents validated and optimized for in vitro selection
- Primers designed to maximize enrichment and reduce cross-reactivity
- Specially designed substrate maximizes fluorescence enhancement while reducing background noise
- In-depth protocols and guides to avoid common pitfalls and maximize success
- Directly compatible with Optional Supplementary kits for Next-Generation Sequencing.
- All ANDzymeTM Starter Kits come with a 10% discount towards NGS primers and sequencing!
NOTE: If you are familiar with the in vitro selection process, our ANDzymeTM
Core Library kit will include the necessary components to begin your experiments. If you need any assistance, do not hesitate to contact us.