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UrasensorsTM

                    
    

Colorimetric reporters are attractive for developing biosensors and diagnostic assays as colourimetric changes can be detected by our eyes. UrasensorsTM  are Urease-DNA conjugates that have been engineered to be used as a reporting platform to modulate the pH of a solution. Changes in pH can then be monitored by a variety of pH-responsive indicators that often produce sharp distinctive colour transitions. The Urease-DNA conjugates can serve to fulfill many functions from capture to recognition. Contact us to see how it can help with your research! Contact Us.

                  

Features and Benefits 



 

       

   

     

Cross-Platform Integration

Converting platforms such as PCR, RCA, DNAzymes and Aptamers to simple colourimetric sensors





 

Easy Interpretation

Changes in colour is clear and unambiguous
 



  Simplicity

No reliance on expensive or complex laboratory equipment


Detection of DNA through Urease Capture  


                  
Urease-DNA conjugates can be easily captured through the conical Watson-Crick base paring. As an example for potential uses of UrasensorsTM, detection of nucleic acids can be achieved by using the target DNA or RNA molecule as a bridge between the Urease-DNA conjugate and an immobilized capture sequence. The capture of Urease will faciliate the modulation of the solution's pH and a colour change can be observed using pH sensitive dyes. 


                    

Coupling of ANDzymes to Detect E. coli


                    
    

By taking advantage of our ANDzyme probes for recognition of bacteria, we can couple Urease-DNA conjugates to various ANDzymes to create a modified Litmus test against a specific pathogen. ANDzymes are RNA-cleaving DNAzymes that cleaves itself into two fragments in the presence of bacterial cells. This cleavage reaction releases Urease conjugates into the solution which can be isolated and transferred to another reaction vessel that contains all the reagents needed to produce a colour change. This assay does not depend on any complex equipment for use and qualitative determination of bacteria is fast and easy.

                  

Implementing Colour to PCR

                  
Polymerase chain reaction(PCR) is an invaluable technique used in molecular biology and biochemistry due to its sensitivity through exponential enrichment of a single copy of DNA. As a result, PCR has been applied in the development of various diagnostic tests using specific genetic markers. The presence of these markers can easily be identified quickly and efficiently by incorporating primers with a short capture sequence. Upon completion of PCR, Urease-DNA conjugates will hybridize with the capture sequence and provide a new utility of converting PCR into a simple colourimetric test.