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Standard Aptamer Selection Buffer

SKU: SB01
Standard buffering, salts, and cofactors used for isolating aptamers
Price: $45.00
Standard Aptamer Selection Buffer
Standard Aptamer Selection Buffer
Product Details
All in vitro aptamer selection experiments require the use of a "selection buffer". This buffer not only serve to maintain the pH of the solution, but also provide the necessary ionic strength and co-factors to allow unique DNA structures to form. In addition, the presence of divalent metal ions may act as co-factors to allow DNA to form more intricate and complex structures. Overall, these ions exist to give the DNA library an opportunity to access a diverse set of structural motifs that can be used for recognition of their intended target. 

List of Materials: 2x Selection Buffer

Quantity:  100 mL

Performance and Quality: Our buffers are made with Ultra pure, sterile-filtered water which has been tested for DNase, RNase, Nickase, and Protease activity. 

Buffer Component (@ 2x):
100 mM Tris-HCl (pH 7.5)
300 mM NaCl
50 mM KCl
10 mM MgCl2

2 mM CaCl2
0.02% Tween-20

Storage
 

All buffers should be stored at 4°C
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